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Regarding static scaffold-free 3D cultures, the most common techniques employed are the forced floating method, the hanging drop method, and the magnetic levitation method while for dynamic systems, agitation-based techniques are applied using spinner flask bioreactors and rotational flask bioreactors [247]

GSH is a tripeptide synthesized from glutamate, cysteine, and glycine in two steps catalyzed by -l-glutamyl-l-cysteine:glycine ligase and glutathione synthetase

The QC samples were all within 2-fold standard deviation ( 2SD) (Figure S3b)

[DOI] [PMC free article] [PubMed] [Google Scholar] 247.Chen X., Wu W., Gong B., Hou L., Dong X., Xu C., Zhao R., Yu Q., Zhou Z., Huang S., et al